α135 tocopherol phosphate  (MedChemExpress)

Journal: Cancer Cell International

Article Title: Polysaccharides from Huaier induce autophagy-dependent ferroptosis to inhibit breast cancer stem cells in triple-negative breast cancer

doi: 10.1186/s12935-025-04102-4

Figure Lengend Snippet: PS-T restrains BCSCs by inducing ferroptosis in TNBC. ( A - B ) Cell viability heatmap of the effects of different cell death inhibitors on PS-T–induced cell death. ALDH + MDA-MB-231 cells were treated with indicated concentration of PS-T with or without Z-VAD-FMK (30 µM), Necrostatin-1 (Necro-1, 20 µM), LDC7559 (5 µM), α-tocopherol (α-toc, 100 µM) or liproxstatin-1 (Lip-1, 200 nM) for 24 h ( n = 9). ( C ) GSEA result of MDA-MB-231 cells treated with or without PS-T using WP_FERROPTOSIS gene set. ( D - F ) The ROS fluorescence ( D ), C11 BODIPY fluorescence ( E ), and relative MDA concentration ( F ) of 0, 10, 20, and 50 µg/mL PS-T–treated ALDH + MDA-MB-231 cells ( n = 9). Scale bars = 20 μm. ( G - H ) The relative MDA concentration ( G ) and C11 BODIPY fluorescence ( H ) of breast cancer tissue of xenograft models treated with or without PS-T ( n = 9). ( D , G , H ) The Student’s t-test was employed. ( E , F ) One-way analysis of variance and post-hoc Bonferroni tests were utilized. Each experiment was repeated three times, and data was pooled. (mean ± standard deviation; *** P < 0.001)

Article Snippet: The total carbohydrate content is 96.5%, as measured by the phenol-sulfuric acid method. α-Tocopherol phosphate (HY-16686, 100 μM) and Erastin (HY-15763, 10 μM) were obtained from MedChemExpress (USA).

Techniques: Concentration Assay, Fluorescence, Standard Deviation

Journal: Cancer Cell International

Article Title: Polysaccharides from Huaier induce autophagy-dependent ferroptosis to inhibit breast cancer stem cells in triple-negative breast cancer

doi: 10.1186/s12935-025-04102-4

Figure Lengend Snippet: Ferroptosis inhibitors reverse the effect of PS-T on BCSCs. ( A - C ) The ROS fluorescence ( A ), C11 BODIPY fluorescence ( B ), and relative MDA concentration ( C ) in ALDH + MDA-MB-231 cells that were treated alone or in combination with PS-T (10 µg/mL) and ferroptosis inhibitors α-tocopherol (α-toc, 100 µM) or liproxstatin-1 (Lip-1, 200 nM) for 24 h ( n = 9). Scale bars = 20 μm. ( D - E ) The mRNA levels of POU5F1, SOX2, and NAONG in MDA-MB-231 cells that treated with PS-T combined with α-toc ( D ) or Lip-1 ( E ) ( n = 9). ( F - G ) The proportion of ALDH + ( up ) and CD44 high CD24 low cells ( down ) in PS-T–treated MDA-MB-231 cells that co-treated with or without α-toc ( F ) or Lip-1 ( G ) ( n = 9). ( H - I ) Colony formation ( H ) ( n = 3) and mammosphere assays ( I ) ( n = 9) of ALDH + MDA-MB-231 cells treated with PS-T, ferroptosis inhibitors, and a combination of both. Scale bars = 50 μm. ( A - I ) One-way analysis of variance and post-hoc Bonferroni tests were utilized. Each experiment was repeated three times, and data was pooled. (mean ± standard deviation; (A-I) One-way ANOVA with Bonferroni’s post-hoc test was used. ** P < 0.01; *** P < 0.001)

Article Snippet: The total carbohydrate content is 96.5%, as measured by the phenol-sulfuric acid method. α-Tocopherol phosphate (HY-16686, 100 μM) and Erastin (HY-15763, 10 μM) were obtained from MedChemExpress (USA).

Techniques: Fluorescence, Concentration Assay, Standard Deviation

Journal: MethodsX

Article Title: Preparation of a universally usable, animal product free, defined medium for 2D and 3D culturing of normal and cancer cells ☆

doi: 10.1016/j.mex.2024.102592

Figure Lengend Snippet: Preparation of the H 2 O solution.

Article Snippet: , α-Tocopherol phosphate , , Sigma-Aldrich , T2020.

Techniques: Concentration Assay, Sterility

Journal: MethodsX

Article Title: Preparation of a universally usable, animal product free, defined medium for 2D and 3D culturing of normal and cancer cells ☆

doi: 10.1016/j.mex.2024.102592

Figure Lengend Snippet: Components stored (aliquoted in Eppendorf tubes together in plastic bags) at −80 °C.

Article Snippet: , α-Tocopherol phosphate , , Sigma-Aldrich , T2020.

Techniques:

Journal: MethodsX

Article Title: Preparation of a universally usable, animal product free, defined medium for 2D and 3D culturing of normal and cancer cells ☆

doi: 10.1016/j.mex.2024.102592

Figure Lengend Snippet: Composition of all non-protein components in the defined medium after supplementation of DMEM/F12 to enrich the medium.

Article Snippet: , α-Tocopherol phosphate , , Sigma-Aldrich , T2020.

Techniques: Concentration Assay